Long-term Multicellular Spheroids Culture and Anti- Cancer Drug Activity Evaluation in a Microfluidic System
نویسنده
چکیده
In this paper, a microfluidic system for long-term multicellular tumor spheroids culture and analysis is presented. Human carcinoma cells were cultured in an array of microwells for four weeks and a slowdown of the proliferation with maintained viability was observed. The construction of the device enabled observation of decreasing spheroid diameter as an indicator of cell death. Long-term observation of cell viability was possible. An activity of repeated doses of 5-fluorouracil was observed.
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